Procedure

How we prepared the sample for the SEM

• We put the carbon tape on the stub 
• We divided the carbon tape into three sections to receive the three different types of pollen
• We took the dried Hemerocallis, Zantedeschia aethiopica, and Lilium asiatica samples that Ms. Lindahl already had and used individual paintbrushes to put the three pollen samples on their sections of the carbon tape.
• We blew the sample with compressed air to blow off any loose pollen grains and to push the pollen grains further onto the carbon tape.

(photo credit to Sophie, model is Liam the Great)

• The software that we used for taking 35x photographs was LeicaEZ4 HD. We put the different samples on petri dishes and then looked at them on the screen of the computer. 
• Then we focused the image, found the pollen in the sample, and zoomed in to 35x. We refocused on the pollen, and took photos of the sample. 
• Then we repeated with another lily sample.

• The software that we used for taking SEM photographs was FEI Phenom Pro. 
• When we took the pictures on the SEM, we were looking for the 3 samples of daylily, asian lily and calla lily pollen to take more in depth look at whether or not our hypothesis is correct. 
• We photographed the pollen at different magnifications to look at the size, shape, and texture of the pollen grains to compare and contrast them to one another.

Addendum 12/20/13

Today, Sophie worked on coloring and posting SEM photos. Liam and I made and posted the table. Cory and I started working on the procedure. 

Pollen Table

Pollen Table

SEM Microscope Pictures

These are photos taken with the FEI Phenom Pro SEM Microscope:

This is a photo taken by me of Asian Lily pollen.

This is a photo taken by me of measurements of Asian Lily pollen.

This is a photo taken by me of the Asian Lily pollen up close.

This is a photo taken by me of Asian Lily pollen at 2000x.

This is a picture taken by me of Calla Lily pollen.

This is a photo taken by me of the measurements of Calla Lily pollen at 2000x.

This is a photo taken by me of the Calla Lily pollen close up.

This is a photo taken by me of the Daylily pollen.

This is a photo taken by me of the measurements of the Daylily pollen.

This is a photo taken by me of the Daylily pollen close up.

This is a photo taken by me of Daylily pollen at 2000x.

Addendum 12/18/13

Today, Liam posted the background information and is starting to make the table, Cory posted the hypothesis, Sophie posted different pictures. We also used the SEM. Cory was the recorder, Sophie was the SEM operator and photographer, and Liam was the data collector.

Hypothesis

• The pollen will all be different. 
• If this is supported, all the pollens will be different with no resemblance to eachother.
• If this is rejected, two or more of the pollens will have resemblance to each other or show signs of a common ancestor.

• The pollen will be similar because they share a common ancestor.
•  If this is supported, two  or more of the pollens will have resemblance to each other or show signs of a common ancestor.
• If this is rejected, all the pollens will be difference with no resemblance to each other.

Edit: December 20th

The first hypothesis is Liam's

The second hypothesis is that of the rest of the group

Photos From Leica Microscope

These are photos taken by the Leica Microscope EZ4 HD, and edited using the Leica imaging 1.0 program.

This is a photo taken by Liam of the Asian Lily anther and pollen.

This is a photo taken by Sapphira of an Asian Lily filament.

This is a photo taken by Sapphira of an Asian Lily petal.

This is a photo taken by Sapphira of a Calla Lily Stigma.

This is a photo taken by Sapphira of a Calla Lily Ovary.

This is a photo taken by Liam of a Calla Lily anther and pollen.

This is a photo taken by Liam of a Daylily anther and pollen.

This is a photo taken by me of our stub. the pollen between 1 and 2 is Calla Lily, between 2 and 3 is Daylily, and between 3 and 1 is Asian Lily.

Background info on the amazing flower of the lily.

Growing from bulbs, lily flowers blossom between spring and autumn, with different varieties opening at different times. They come in a variety of colors, including white, yellow, orange, red, pink, violet and blue. Some flowers are speckled or spotted. These flowers often have a trumpet shape, with showy petals that curl outwards and a drooping style. Their stamen and pistils are prominent.

Lilium flowers need well-drained soil with lots of organic matter, which enriches the soil with nutrients and keeps it moist. In cool climates, full sun is best, but lilies can tolerate partial shade in warm places. In addition to regular watering.

Information cred: 
http://homeguides.sfgate.com/information-lily-flowers-44001.html
http://www.flowers.org.uk/flowers/flowers-names/i-l/lily/
http://www.aboutflowers.com/flower-a-plant-information-and-photos/flowers/lily.html

Team Agreement

Liam has set up and designed the blog. He will also be writing the background to help us have a strong hypothesis.

Sophie has posted our question. She will also be posting different pictures throughout the lab to show what we are doing. Her pictures will show all of the steps we are taking and different samples with captions stating what it is. 

Sapphira is in charge of writing the team agreement.

Cory is in charge of writing the hypothesis.

Cory and I will also be working together to post anything else we feel as though we need to go over or need to be posted.

Question

Is there a difference between the pollen of Day-, Calla, and Asian Lilies?